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Trial Title:
Cytokine Regulation of Natural Killer Receptors in Inhibiting Activated T Cell Function
NCT ID:
NCT00173290
Condition:
Cervical Cancer
Breast Cancer
Endometrial Cancer
Cancer
Conditions: Official terms:
Uterine Cervical Neoplasms
Endometrial Neoplasms
Conditions: Keywords:
Cervical cancer
Breast cancer
T lymphocyte
NK receptor
Study type:
Observational
Overall status:
Unknown status
Summary:
In this study proposal, the investigators will extend their previous studies and examine
the kinetic cytotoxic activity with concordant expression of inhibitory natural killer
(NK) receptors (iNKR) on activated T cells. The inhibitory role of cytokines will be
defined by utilizing the investigators' previously established models of mixed
lymphocytes and tumor cells coculture to analyze the expression and activity of cytokines
involved in the regulation of iNKRs on cancer-encountered T cells.
Detailed description:
In our extended studies, we have directly examined the expressions of various inhibitory
natural killer cell Receptors (iNKRs) on Tumor-infiltrating lymphocytes (TILs) derived
from human Cervical cancer (CC) by triple-color flow cytometry with combination of
different surface markers. We found up-regulated expression of certain iNKRs (CD94/NKG2A)
in TILs and in mixed lymphocyte-cancer cell cocultures. In our previous studies, we
demonstrated that certain cytokines, including IL-10, TGF-beta (Sheu et al, Journal of
Immunology, 2001, 167:2972-2978), and IL-15 (Sheu et al, Cancer Research, 2005,
65:2921-2929) can be expressed by CC cells. We further demonstrated that activated T
cells bear iNKRs which inhibit cytotoxic activity. iNKRs are proposed to restrain the T
cell receptor (TCR)-mediated cytolysis. We found that CC cells had altered HLA-A, -B, and
-C molecules in a cancer microenvironment. The acquisition of both NK-like activity and
expression of iNKRs by these T cells is parallel to prevent damage to normal host cells.
However, there is also limited knowledge about the regulatory role of iNKR expression in
T cell cytotoxicity.
Criteria for eligibility:
Criteria:
Inclusion Criteria:
- Cancer patients
- Healthy volunteers
Exclusion Criteria:
- Immunosuppression
- HIV carrier
Gender:
Female
Minimum age:
18 Years
Maximum age:
50 Years
Healthy volunteers:
Accepts Healthy Volunteers
Locations:
Facility:
Name:
National Taiwan University Hospital
Address:
City:
Taipei
Zip:
100
Country:
Taiwan
Status:
Recruiting
Contact:
Last name:
Bor-Ching Sheu, MD, PhD
Phone:
886-2-2312-3456
Phone ext:
5559
Email:
bcsheu@ha.mc.ntu.edu.tw
Start date:
July 2004
Completion date:
December 2006
Lead sponsor:
Agency:
National Taiwan University Hospital
Agency class:
Other
Source:
National Taiwan University Hospital
Record processing date:
ClinicalTrials.gov processed this data on November 12, 2024
Source: ClinicalTrials.gov page:
https://clinicaltrials.gov/ct2/show/NCT00173290
