Safety and Efficacy Study of Virus Activated Killer Immune Cells (VAK) for Malignant Pleural and Peritoneal Effusion

Trial Title: Safety and Efficacy Study of Virus Activated Killer Immune Cells (VAK) for Malignant Pleural and Peritoneal Effusion

NCT ID: NCT05565014

Condition: Malignant Pleural Effusion
Malignant Peritoneal Effusion

Conditions: Official terms:
Pleural Effusion, Malignant
Pleural Effusion

Study type: Interventional

Study phase: Early Phase 1

Overall status: Unknown status

Study design:

Allocation: Randomized

Intervention model: Parallel Assignment

Primary purpose: Treatment

Masking: None (Open Label)

Intervention:

Intervention type: Drug
Intervention name: Virus Activated Killer immune Cells
Description: The VAK made from Pleural Effusion should be given to treat Malignant Pleural Effusion.The VAK made from Peritoneal Effusion should be given to treat Malignant Peritoneal Effusion.
Arm group label: Virus Activated Killer immune Cells（VAK）

Other name: VAK

Intervention type: Drug
Intervention name: Saline
Description: The use of saline was the same as VAK group.
Arm group label: saline

Summary: Theory of VAK： 1. Immune cells (T cells for example) of cancer subjects may be domesticated by the tumor microenvironment, and have low efficacy to kill cancer cells. They could be restimulated by virus antigen, and play a powerful tumor killing role while intrapleural to subjects. 2. Releasing of tumor-associated antigen could induce specific anti-tumor immune response. Preparation of VAK： 1. Separate the immune cells and tumor cells from Malignant Pleural and Peritoneal Effusion. 2. Incubate the immune cells with inactivated viruses and tumor cells. 3. Wash to remove impurities. 4. Intrapleural the immune cells to patients

Detailed description: There are a large number of immune cells in immune tolerance state in the tumor microenvironment. The theoretical basis of this clinical study is to use ultraviolet inactivated oncolytic herpes simplex virus type 2 (UV-oHSV2) to activate PBMC or immune cells in immune tolerance state in malignant pleural and peritoneal fluid in vitro, and to transfusion the activated immune cells back to the patient's peripheral blood or pleural and peritoneal fluid to kill tumor cells in malignant pleural and peritoneal fluid to further control the volume of malignant pleural and peritoneal fluid. Our study indicated that UV-oHSV2 potently activated human peripheral blood mononuclear cells leading to increased antitumor activity in vitro and in vivo. We also found that UV-oHSV2 could induce NK cells (isolated from healthy donors' PBMC or patient pleural effusion) proliferation, secretion of IFN-γ. We further found that UV-oHSV2 could enhance NK cells (isolated from healthy PBMC or patient pleural effusion) antitumor ability in vitro and in vivo. Based on the above research, we carried out an open, single center, prospective clinical trial to evaluate the safety and effectiveness of VAK cells in the treatment of malignant pleural and peritoneal effusions. The detailed protocol as follow: Isolation of lymphocytes from the patient's pleural effusions or ascites: 1. Add pleural fluid into a 50ml centrifuge tube, centrifuge at 400g, and discard the supernatant. 2. Resuspend the precipitate with 40ml of normal saline and pass through 70 μ M U.M and then rinse the cell filter once with 5ml physiological saline. 3. Discard the cell filter, and centrifuge the filtered cell suspension in the centrifuge tube at 400g for 5min. After centrifugation, discard the supernatant and resuspend the cell pellet with 5ml of normal saline. 4. Mix Ficoll upside down before use, take a new 15ml centrifuge tube and add 5ml Ficoll (suck with syringe). 5. Gently add 5-6ml cell suspension onto Ficoll layer. 6. Centrifuge 400g, 30-40min, 18 ℃ - 20 ℃, the centrifuge needs to be slowly raised and lowered, and the sudden stop braking setting should be closed. 7. Take the middle white membrane layer (lymphocytes) into a new centrifuge tube. 8. Add 3 times the volume of normal saline to the absorbed lymphocytes, resuspend the cells, centrifuge 400-500g, 10-15min, 18 ℃ - 20 ℃. 9. Repeat washing once, and the final precipitate is resuspended with 5ml serum-free medium (RPMI1640). 10. Take 20 μ L of cell suspension in EP tube, add equal volume of trypan blue staining solution, gently blow and count with cell counter. 11. In the process of planting the isolated immune cells, the cell culture density was controlled within the range of 106, and 10% autologous plasma was added. 12. Take 4ml of cell suspension for separate culture, and add the corresponding amount of inactivated virus to the remaining cell suspension with the infection number MOI = 1. The calculation formula is as follows: volume of uv-oh2 added = (1 × Number of cells added per well plate or bottle / oh2 virus titer) 13. After the immune cells are planted, they are placed in a 5% CO2 incubator for 36h-48h. After cell culture, 30-50ML of prepared lymphocytes (qualified by PCR sterility test and endotoxin test) were infused into the chest and abdominal cavity of the patient.

Criteria for eligibility:
Criteria:
Inclusion Criteria: 1. Willing to sign informed consent; 2. Pathological confirmed advanced malignant tumor with malignant pleural or peritoneal effusion; 3. Vital signs stable, Eastern Cooperative Oncology Group (ECOG) Performance Status 0-2; 4. Age ≥ 18 years old, gender unlimited; 5. Expected survival period more than 3 months; 6. Subjects agree to take effective contraceptive measures at the time of enrollment and within 4 months after enrollment. The pregnancy test of female patients must be negative; 7. Sufficient bone marrow, liver and kidney functions. Laboratory tests within 7 days before the first drug use meet the following requirements: - Coagulation function: APTT ≤ 1.5 × ULN, while INR or PT ≤ 1.5 × ULN (not receiving anticoagulant therapy); ② Blood routine examination: Hgb ≥ 80g / L, WBC > 3 × 10^9/L、NEU≥1.0 × 10^9/L、PLT≥80 × 10^9/L; - Liver function: total bilirubin ≤ 1.5 times the upper limit of normal value (ULN); AST and alt ≤ 2.5 times ULN (if abnormal liver function is mainly caused by tumor infiltration, it can be ≤ 5 times ULN; alkaline phosphatase ≤ 2.5 times ULN; ④ Renal function: bun and Cr ≤ 1.5 times ULN, creatinine clearance ≥ 40ml / min (calculated by Cockcroft Gault formula). Exclusion Criteria: 1. Subjects requiring emergency treatment due to intestinal obstruction or vascular compression; 2. Subjects with active hemolytic anemia; 3. Subjects with active central nervous system metastases were excluded, except those with brain metastases or asymptomatic cancer cells found in cerebrospinal fluid; 4. Pregnant or lactating female; 5. Systemic active infection, serious coagulation disorder or serious heart, respiratory and immune system diseases; 6. Congenital or acquired immune function defects (such as HIV infection), hepatitis B infection (HBV-DNA ≥ 10 ^ 4 / ml), hepatitis C infection (HCV antibody and HCV RNA positive); 7. Subjects who had serious infection within 4 weeks before the first medication were excluded, including but not limited to infectious complications, bacteremia and severe pneumonia requiring hospitalization; 8. Subjects with active autoimmune diseases or a history of autoimmune diseases that may recur, but the following are not excluded: - Type 1 diabetes - Hypothyroidism (if controlled by hormone replacement therapy only) - Controlled celiac disease ④ Skin diseases without systemic treatment (such as vitiligo, psoriasis, alopecia) ⑤ Any other disease that does not recur without external triggers. 9. Those who had severe hypersensitivity to the drugs used in this protocol in the past; 10. Subjects who are ready for or have previously received tissue / organ transplantation; 11. Subjects with large pericardial effusion were excluded; 12. Exclude those who have suffered from other malignant tumors within 2 years, except for cervical carcinoma in situ, low-risk gastrointestinal stromal tumor, skin basal cell carcinoma, skin squamous cell carcinoma, thyroid papillary carcinoma and breast ductal carcinoma in situ that have been effectively removed; 13. Exclude subjects who have been vaccinated or will be vaccinated with live vaccine within 4 weeks before the first administration; 14. Other circumstances that the investigator considers inappropriate for clinical trials.

Gender: All

Minimum age: 18 Years

Maximum age: N/A

Healthy volunteers: No

Locations:

Facility:
Name: Hubei Cancer Hospital

Address:
City: Wuhan
Zip: 430079
Country: China

Status: Recruiting

Contact:
Last name: Sheng Hu, MD

Phone: 15377602179
Email: ehusmn@163.com

Contact backup:
Last name: Shuang Dong, UK

Phone: 15802766720
Email: dongshuang_2008@163.com

Start date: July 24, 2020

Completion date: June 30, 2024

Lead sponsor:
Agency: Sheng Hu
Agency class: Other

Collaborator:
Agency: Binhui Biopharmaceutical Co., Ltd.
Agency class: Industry

Source: Hubei Cancer Hospital

Record processing date: ClinicalTrials.gov processed this data on November 12, 2024

Source: ClinicalTrials.gov page: https://clinicaltrials.gov/ct2/show/NCT05565014