The Study of Anti-CD19 CAR NK Cells in the Treatment of Relapsed/Refractory Diffuse Large B Cell Lymphoma

Trial Title: The Study of Anti-CD19 CAR NK Cells in the Treatment of Relapsed/Refractory Diffuse Large B Cell Lymphoma

NCT ID: NCT05673447

Condition: Diffuse Large B Cell Lymphoma

Conditions: Official terms:
Lymphoma
Lymphoma, B-Cell
Lymphoma, Large B-Cell, Diffuse

Conditions: Keywords:
B-Cell Lymphoma
CD19
CAR-NK
diffuse diffuse large B cell lymphoma

Study type: Interventional

Study phase: Early Phase 1

Overall status: Recruiting

Study design:

Allocation: N/A

Intervention model: Single Group Assignment

Primary purpose: Treatment

Masking: None (Open Label)

Intervention:

Intervention type: Biological
Intervention name: anti-CD19 CAR NK cells
Description: Patients will receive Fludarabine (30 mg/m2 per day) and Cyclophosphamide (300mg/m2 per day) on day -5, -4, and -3. Doses of 6×10^8, 1×10^9, 1.5×10^9 Anti-CD19 CAR NK cells will infused in each group using the "3 + 3" dose-escalation strategy.
Arm group label: anti-CD19 CAR NK cells

Summary: A single arm, open-label pilot study is designed to determine the safety and effectiveness of anti-CD19 CAR NK cells in patients with B-cell Non Hodgkin Lymphoma. 9-12 patients are planned to be enrolled in the dose-escalation trial (6×10^8 cells, 1×10^9 cells, 1.5×10^9 cells). The primary endpoints are DLT, MTD. The secondary endpoints are the overall response rates (ORR) and disease control rate (DCR).

Criteria for eligibility:
Criteria:
Inclusion Criteria: 1. Subjects voluntarily participate in this clinical study and sign the Informed Consent Form (ICF). 2. Clinical diagnosis of CD19 positive diffuse large B cell lymphoma as defined by the 2017 World Health Organization (WHO) classification of tumors of haematopoietic and lymphoid tissue. 3. Relapsed/Refractory diffuse large B cell lymphoma refers to: not complete response (CR) of 2 lines of standard treatment; PD after treatment or duration of SD less than 6 months after treatment; progress or relapse within 12 months after autologous stem cell transplant. 4. Subjects with a measurable or evaluable lesion (more than one lesion≥15mm) according to IWG criteria. 5. Age≥ 18 years old and ≤ 75 years old, male or female. 6. Subjects with estimated survival > 12 weeks. 7. Serum albumin (ALB) ≥30g/L, Total Bilirubin (TBIL) ≤ 25.7μmol/L, serum creatinine (SCr) ≤ 132.6μmol/L, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) ≤ 3 times the upper limit of normal (ULN). 8. Absolute neutrophil count (ANC) ≥ 1.0×109/L, platelet count ≥ 50×109/L. 9. ECOG performance ≤ 1. 10. Left ventricular ejection fraction (LVEF) ≥50% and no clinically significant pericardial effusion. 11. ≥ 4 weeks after subjects received last dose treatment (Radiotherapy, chemotherapy, monoclonal antibody therapy or other treatments). Exclusion Criteria: 1. Subjects with known severe allergic reactions, hypersensitivity, contraindication to any medications during the trial (cyclophosphamide, fludarabine, tozumabs), or subjects with a history of severe allergic reactions. 2. Relapsed after allogenic haemopoietic stem cell transplantation (HSCT). 3. Subjects with active infection receiving intravenous (IV) antibiotic treatment, or received intravenous (IV) antibiotic treatment within one week prior to anti-CD19 CAR NK Cell infusion. 4. Subjects with acquired and congenital immunodeficiency diseases. 5. Subjects with grade III or IV heart failure (NYHA classification). 6. History of epilepsy or other central nervous system (CNS) diseases. 7. Subjects with extranodal lymphoma in Intracranial, lung, or gastrointestinal tract. 8. History of other primary malignant tumors except: 1. Cured non-melanoma skin cancer by surgical excision, for example basal cell carcinoma (BCC); 2. Cured primary malignant tumors, such as cervical cancer, superficial bladder cancer, breast cancer. 9. Systemic corticosteroids are used concomitantly within 2 weeks prior to treatment. 10. Females who are pregnant, lactating, or planning a pregnancy within six months. 11. Subjects who have received other clinical trial treatment within 3 months. 12. Any situation judged by the investigators that may increase the risk of the subjects or interfere with the clinical trial outcome.

Gender: All

Minimum age: 18 Years

Maximum age: 70 Years

Healthy volunteers: No

Locations:

Facility:
Name: Changhai Hospital

Address:
City: Shanghai
Country: China

Status: Recruiting

Contact:
Last name: Jianmin Yang, Doctor

Phone: 86-18317172636
Email: yangjianmin@csco.org.cn

Start date: March 1, 2023

Completion date: December 30, 2024

Lead sponsor:
Agency: Changhai Hospital
Agency class: Other

Collaborator:
Agency: Nanjing Enricnk Biotech Co., Ltd
Agency class: Other

Source: Changhai Hospital

Record processing date: ClinicalTrials.gov processed this data on November 12, 2024

Source: ClinicalTrials.gov page: https://clinicaltrials.gov/ct2/show/NCT05673447