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Trial Title:
Single-Cell Transcriptomics of the Peritoneal Microenvironment of Colorectal PC
NCT ID:
NCT05676372
Condition:
Peritoneal Metastases
Conditions: Official terms:
Neoplasm Metastasis
Study type:
Observational
Overall status:
Recruiting
Study design:
Time perspective:
Prospective
Intervention:
Intervention type:
Procedure
Intervention name:
CRC+HIPEC
Description:
Sample prelevation of cytoreductive surgery
Summary:
Single cell transcriptomics of CRC PC samples
Detailed description:
Research methodology
Before inclusion of patients, patients will be screened for the presence of hepatitis B,
hepatitis C and HIV to ensure researcher safety. During cytoreductive surgery, a total of
five samples of tumoral tissue will be acquired. Three of those samples will be utilized
for ScRNASeq analysis, and two samples of a macrometastasis will be snap frozen and
cryopreserved.
Single-cell RNA-Sequencing analysis We will comprehensively catalog the stromal cell
types in the microenvironment of colorectal cancer peritoneal metastases utilizing
single-cell RNA sequencing (scRNAseq) on three samples (one of which should preferably
originate from the omentum majus, to encompass milky spots) acquired from surgical waste
material of patients undergoing cytoreductive surgery. After sample prelevation, samples
will be transported as soon as possible while cooled and submerged in 10% PBS to the VIB
facilities, where single cell suspensions will be made according to standard protocols.
After cell selection by FACS (CD45+ and live/dead stain, to ensure a 50/50 mix of immune
and stromal cells) and library prep, ScRNASeq will be performed.
Single cell sequencing analysis will allow us to identify cell clusters that by usage of
marker genes will be assigned to different known cell lineages. After annotation of data,
a "landscape" of the composition of the tumor stroma will be acquired.
Criteria for eligibility:
Study pop:
Patients undergoing CRS+HIPEC
Sampling method:
Non-Probability Sample
Criteria:
Inclusion Criteria:
- Patients undergoing CRS+HIPEC for PC of colorectal origin
Exclusion Criteria:
- Previous HIPEC/PIPAC
Gender:
All
Minimum age:
N/A
Maximum age:
N/A
Healthy volunteers:
No
Locations:
Facility:
Name:
UZ Ghent
Address:
City:
Ghent
Zip:
9000
Country:
Belgium
Status:
Recruiting
Contact:
Last name:
Wim P Ceelen, MD,PhD
Phone:
093326251
Phone ext:
+32
Email:
wim.ceelen@ugent.be
Investigator:
Last name:
Jesse G Demuytere, MD
Email:
Sub-Investigator
Start date:
June 16, 2020
Completion date:
December 31, 2025
Lead sponsor:
Agency:
University Hospital, Ghent
Agency class:
Other
Collaborator:
Agency:
Flemish institute of biotechnology (VIB)
Agency class:
Other
Source:
University Hospital, Ghent
Record processing date:
ClinicalTrials.gov processed this data on November 12, 2024
Source: ClinicalTrials.gov page:
https://clinicaltrials.gov/ct2/show/NCT05676372
