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Trial Title:
FOS Immunohistochemical Staining of Colorectal Cancer and Its Adjacent Tissues
NCT ID:
NCT05698082
Condition:
Colorectal Cancer
Monkey Pox
Conditions: Official terms:
Mpox (monkeypox)
Colorectal Neoplasms
Conditions: Keywords:
Differential expression of gene
Immunohistochemical staining
Tumor microenvironment
Study type:
Interventional
Study phase:
Phase 2/Phase 3
Overall status:
Recruiting
Study design:
Allocation:
N/A
Intervention model:
Single Group Assignment
Primary purpose:
Diagnostic
Masking:
None (Open Label)
Intervention:
Intervention type:
Diagnostic Test
Intervention name:
Immunohistochemical staining with anti-FOS
Description:
The tissue sections were incubated with anti-FOS (promab 30360) at 4ºC overnight and
washed three times with PBS. After that, it is required that incubation with secondary
anti-peroxidation sunflower at 37ºC for 30 minutes. After washing three times again with
PBS, the sections were developed in diaminobenzidine and microscopic images were made by
light microscopy.
Arm group label:
Test group-stained by FOS antibody
Summary:
Colorectal cancer tissue sections were obtained according to the inclusion criteria. The
formalin was used to immersed all cancer specimens. And tissues were cut to 5 μm
thickness and placed on glass slides before staining. Endogenous peroxidase activity was
inhibited and blocked by de-paraffinizing, rehydrating, and using 5% bovine serum albumin
at 37ºC for 30 min. The treated sections were incubated with anti-FOS (promab 30360) at
4ºC overnight and washed three times with PBS. After that, it is required that incubation
with secondary anti-peroxidation sunflower at 37ºC for 30 minutes. After washing three
times again with PBS, the sections were developed in diaminobenzidine and microscopic
images were made by light microscopy.
Criteria for eligibility:
Criteria:
Inclusion Criteria:
- (a) patients with histologically confirmed CRC (b) patients who underwent resection
of the primary tumor (c) patients at first visit who had not received treatment
before blood testing.
Exclusion Criteria:
- (a) familial adenomatous polyposis or hereditary colon cancer (b) no signs of
clinical infection, such as fever, on the day of blood collection; And (c) patients
with multiple primary tumors during hospitalization.
Gender:
Male
Minimum age:
18 Years
Maximum age:
75 Years
Healthy volunteers:
No
Locations:
Facility:
Name:
Guangxi Medical University Cancer Hospital
Address:
City:
Nanning
Country:
China
Status:
Recruiting
Contact:
Last name:
Xiangliang Huang, Dr.
Phone:
+8613617713823
Email:
xiaoliang@sr.gxmu.edu.cn
Start date:
December 1, 2020
Completion date:
January 1, 2025
Lead sponsor:
Agency:
xiaoliang Huang
Agency class:
Other
Source:
Cancer Hospital of Guangxi Medical University
Record processing date:
ClinicalTrials.gov processed this data on November 12, 2024
Source: ClinicalTrials.gov page:
https://clinicaltrials.gov/ct2/show/NCT05698082
