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Trial Title:
Immunohistochemical Evaluation of DNA Methylation and Histone Modification in H. Pylori Associated Gastric Carcinoma
NCT ID:
NCT05811546
Condition:
Gastric Carcinoma
Conditions: Official terms:
Carcinoma
Stomach Neoplasms
Study type:
Observational
Overall status:
Recruiting
Study design:
Time perspective:
Cross-Sectional
Intervention:
Intervention type:
Diagnostic Test
Intervention name:
Immunohistochemical staining for DNMT1 and EZH2
Description:
Immunohistochemical staining for DNMT1 and EZH2 using rabbit monoclonal/polycolonal
antibodies with immunoperoxidase staining will be used as a visualization method.
Antibodies dilutions, antigen retrieval methods, and incubation time will all be
conducted according to the manufacturer's instructions.
Arm group label:
H. pylori Gastritis specimens
Summary:
The goal of this cross-sectional study is to evaluate epigenetic modifications in gastric
carcinogenesis, mainly in H. pylori gastritis vs gastric carcinoma. The main question[s]
it aims to answer are:
- How does H. pylori infection cause gastric cancer?
- Can DNA methylation and other epigenetic changes predict and affect the development
of gastric cancer? Formalin-fixed-paraffin-embedded tissue specimens will be
obtained and reviewed for comparison between epigenetic changes in H. pylori
gastritis and gastric adenocarcinoma.
Criteria for eligibility:
Study pop:
Formalin-fixed-paraffin-embedded tissue blocks from resected specimens and endoscopic
guided biopsies of intestinal type gastric carcinoma, H. Pylori associated gastritis, and
non-H. Pylori gastritis.
Sampling method:
Probability Sample
Criteria:
Inclusion Criteria:
- Resected specimens and endoscopic guided biopsies of intestinal type gastric
carcinoma, H. Pylori associated gastritis, and non-H. Pylori gastritis with:
- Available full clinical data: (age, sex, complaint, findings in clinical
examination).
- Full data of previous endoscopy and radiology.
Exclusion Criteria:
- Cases of diffuse type gastric carcinoma.
- Any case of gastric cancer, H. Pylori associated gastritis, non-H. Pylori gastritis,
benign gastric lesions with missing clinical, endoscopic, radiologic or follow-up
data.
- Cases with no available H&E stained slides or formalin fixed paraffin embedded
block.
Gender:
All
Minimum age:
30 Years
Maximum age:
100 Years
Healthy volunteers:
No
Locations:
Facility:
Name:
Assiut University
Address:
City:
Assiut
Zip:
71515
Country:
Egypt
Status:
Recruiting
Contact:
Last name:
Mahmoud Sherif, Phd
Phone:
+20 1002763939
Email:
m.farouk.pathology@aun.edu.eg
Start date:
May 1, 2022
Completion date:
December 31, 2024
Lead sponsor:
Agency:
Assiut University
Agency class:
Other
Source:
Assiut University
Record processing date:
ClinicalTrials.gov processed this data on November 12, 2024
Source: ClinicalTrials.gov page:
https://clinicaltrials.gov/ct2/show/NCT05811546
