Vemurafenib appears effective in hairy cell leukemia31 Oct 2015
A short course of oral vemurafenib (Zelboraf) was found to be highly effective in patients with relapsed or refractory hairy cell leukemia, producing remarkable response rates with low-grade toxic effects, two single-group multicenter studies have shown. The two separate, phase 2 studies were conducted in Italy and in the U.S.
Enrico Tiacci, MD, Centro di Ricerca Emato-Oncologico, University of Perugia, Italy and colleagues wrote in The New England Journal of Medicine wrote in The New England Journal of Medicine: "we found that the V600E mutation of BRAF, a kinase commonly mutated in solid tumors, was the key genetic lesion of hair-cell leukemia...and that BRAF inhibitors in vitro reverse the unique molecular signature and morphologic characteristics of hairy cells and potentially induce their apoptosis, thus establishing BRAF V6600E as an attractive therapeutic target in hairy-cell leukemia."
Clinical design and results of the Italian study
In the Italian trial, 28 patients with disease that was refractory to a purine analogue received vemurafenib at a dose of 960 mg twice a day for a minimum of 8 weeks. Those who did not achieve a complete response at 8 weeks were treated for a maximum of 16 weeks.
After analyzing the results, Tiacci and colleagues observed that:
- 26 out of 28 patients completed the planned treatment with a median duration of 16 weeks (range 8-20 weeks).
- The median follow-up of the 25 patients in the Italian study who responded to vemurafenib was 23 months after the last dose of vemurafenib was given and the median relapse-free survival was 9 months.
- The median treatment-free survival was 21.5 months in all 26 patients who could be evaluated in the Italian trial but it did not differ significantly between those who achieved a complete response and those who achieved a partial response
- 35% of patients had a complete response after a median of 8 weeks of treatment with vemurafenib and 62% of the Italian group achieved a partial response.
- Relapse-free survival was significantly longer among patients who had a complete response than among those who had a partial response.
Only about half of patients in the Italian study could be evaluated for the same endpoint but hairy cells again showed persistent expression of phosphorylated ERK despite prolonged ongoing exposure to vemurafenib.
Clinical results of the U.S. study
The U.S. trial is still ongoing but 26 out of a planned 36 patients have been enrolled and again received vemurafenib at a dose of 960 mg twice a day on a continuous schedule for 12 weeks.
Among survivors in the U.S. trial at the time of data cutoff:
- The median follow-up from the first dose of vemurafenib was 11.7 months
- At one year, the rate of progression-free survival among U.S. patients was 73%
- The rate of overall survival was 91%
- Disease progression occurred in 7 out of 24 U.S. patients or 29% of the group including three patients who had achieved a complete response
- 42% of patients achieved a complete response after 12 weeks of treatment while 58% of patients achieved a partial response
- An overall response rate of 100% was achieved after a median treatment course of 12 weeks.
"A longer duration of treatment did not appear to improve the type or duration of the response," Tiacci and colleagues noted. They added that “retreatment with vemurafenib at the time of relapse was effective in the two trials, although to a lesser extent in the patients who had a relapse after a partial response than in those who had a relapse after a complete response in the Italian study."
As investigators caution, residual hairy cells in bone marrow with their associated BRAF V600E allele burden were consistently present at the end of treatment in the U.S. trial, even after patients achieved a complete response.
The results of both studies reveal that the complete and partial response rates were similar in the two trials.
What about the adverse events?
In both trials, adverse events were mostly grade 1 or 2 and all of them were reversible. However, they included toxic events of the skin, especially rash and photosensitivity, arthralgia or arthritis, pyrexia, and elevated bilirubin. In particular:
- Two patients in the Italian study developed basal-cell carcinomas
- one patient developed a superficial melanoma during treatment
- three patients in the U.S. study developed squamous-cell carcinomas
- One patient developed a basal-cell carcinoma.
"All of these tumors were managed by simple excision," investigators note. In the Italian trial, the dose of vemurafenib was reduced for at least 3 weeks to 720 mg, twice a day or less in 58% of patients while half of patients in the U.S. trial similarly required a dose reduction to 720 mg twice a day or less.
"Vemurafenib can be an ideal salvage treatment for patients"
"This finding suggests that, in at least some patients, leukemic cells develop alternative mechanisms for reactivating MEK and ERK to bypass BRAF blockade," study author observed. Nevertheless, study authors concluded that the absence of clinically significant myelotoxic effects makes vemurafenib an ideal salvage treatment for patients with multiply relapsed hairy cell leukemia who have pancytopenia and scarce bone marrow reserve owing to previous chemotherapies.
According to David Steensma, MD, senior physician, Dana Farber Cancer Institute, Boston, Massachusetts, existing treatments are really very good for the initial treatment of hairy cell leukemia but there is still room for new and effective agents for relapsed/refractory disease. "I think the purine analogues will be with us for the foreseeable future as first-line therapy as they are less expensive than vemurafenib and they are highly effective," Steensma observed.Source: MedPage Today